The Flow CAST® is intended for the determination of basophil activation in patient whole blood samples by flow cytometry. Our unique marker combination – CCR3 and CD63 – combines the simple gating properties for basophil detection by CCR3 with the solid application of the proven activation marker CD63 in one. Thanks to its easy protocol and smart marker combination the first results can be obtained within one hour – perfect conditions for routine laboratories.

CCR3 as Basophil Marker

Basophils constitutively express the eotaxin receptor CCR3 allowing for a robust and accurate selection of cells. CCR3 is detected with a PE-fluorescence labelled monoclonal antibody. This gating of basophil cells is significantly easier than with any other commercially available BAT.

CD63 as Activation Marker

Upon allergen contact, sensitized basophils degranulate and express the cell surface marker CD63.  It  is detected by a FITC-fluorescence labeled monoclonal antibody. CD63 is currently the best-validated basophil activation marker directly linked to basophil degranulation.

Controls 

The first control, anti-FcEpsilonRI, is a highly specific monoclonal antibody binding to IgE receptor. The second control, a tripetide (fMLP) activates the basophils by a non-immunological way.

CD203c as Activation Marker 

The CD203c is an other basophil activation marker for allergy detection after CD63. Our CD203c Reagent Set (RUO) is intended for the use within an extended protocol for the Flow CAST® kit, allowing the measurement of both activation markers, CD63 and CD203c, with two different fluorescence labelled antibodies.

Flow CAST® Product Information  

Method: Flow Cytometry

Time to Result: 1 h (approx.)

Sample Type: 50 µl EDTA blood

Marker: CCR3-PE / CD63-FITC

Order Code: FK-CCR (100 test)

Patents: US 8,563,258 B2; EP 2 037 269 B1

CE (IVDR compliant)

 

Flow CAST®+ CD203C REAGENT SET Product Information 

Method: Flow Cytometry              

Time to Result: 1 h (approx.)         

Sample Type: 50 µl EDTA blood               

Marker: CCR3-PE / CD63-FITC / CD203c-PE-DY647                   

Order Code:  FK-CCR + B-CCR-203SET (each 100 test) 

Research Use Only

Take your Lab Flexibility to the next Level

As a manufacturer of reagents, we have a great understanding of the automation needs faced by end-users when handling diagnostic products on a daily basis.

While there are automation companies with great engineers, we still believe they sometimes lack a full understanding of the daily lab routine.

Understanding these needs encouraged us to create state-of-the-art instruments. Therefore, our company is established to develop products that adapt to the laboratory reality. Our systems are designed to fullfil the highest usability requirements, making our instruments practical and easy to use.

Since its launch, the HELMED® has never stopped evolving, revolutionizing the way lab routine is performed across 5 continents.

Innovative Design – Precision and Standardization faster than ever

Due to its innovative design, e.g. the 3-needle system and 3 independent rings, the HELMED® needs less processing time and increases the reproducibility of results compared to manual analysis or one-needle-systems.

Sample automated Traceability

The HELMED® was the first machine to introduce a built-in barcode reader allowing automated detection and scanning of sample tube IDs, eliminating transcription errors. Communication between LIS and instrument can be done directly or via our middleware HERA®.

Instrument Loading – Step by Step

The loading wizard provides clear visual instructions how to load the required reagents. All the steps after loading can be followed real time.

The QuikRead go Instrument is a compact, fully automatic instrument which is used for quantitative and qualitative measurements together with QuikRead go reagent kits. The QuikRead go system – consisting of the instrument and ready-to-use kits – is specially designed for use in primary healthcare settings. The instrument can be connected to HIS/LIS systems.

Generally

Traceability of test results ensures high quality and reliability. The instrument’s user interface enables a variety of settings to be personalised. There is a choice of 23 different languages, it is easy to add patient and/or operator identification information and the instrument can be easily combined with accessories, such as a barcode reader and a printer.

Ease of use combined with rapid results and reliability makes the QuikRead go system a valuable everyday diagnostic tool for healthcare professionals.

QuikRead go Instrument provides you with

Ease of use

  • Intuitive user interface
  • Interactive touch screen which guides users using animations

Minimal hands-on time

  • Automatic mixing and reagent addition in prefilled cuvettes
  • Results are automatically stored in the instrument’s internal memory, user and/or patient ID can be linked to the results

Connectivity

  • Three standard USB ports enable the use of memory sticks, a printer and a barcode reader
  • LIS/HIS connectivity

This version of QuikRead go Instrument is not registered in the USA (link to the US product version: QuikRead go Instrument for the USA).